CDK4와 hTERT를 도입한 HPV16 E6/E7 불멸화 세포의 확립
Establishment of CDK4/hTERT-Transfected HPV16 E6/E7 Immortalized Cell Lines
박영진, 홍두표, 김은철, 오봉경, 김진,
소속 상세정보
박영진 ( Park Young-Jin ) - 연세대학교 치과대학 구강병리학교실
홍두표 ( Hong Doo-Pyo ) - 연세대학교 치과대학 구강병리학교실
김은철 ( Kim Eun-Cheol ) - 원광대학교 치과대학 구강병리학교실
오봉경 ( Oh Bong-Kyeong ) - 연세 암 센터
김진 ( Kim Jin ) - 연세대학교 치과대학 구강병리학교실
KMID : 0829220100340010027
Abstract
Human papillomavirus (HPV) has been classified as one of the causing factors of head and neck squamous cell carcinoma (HNSCC). However, little is known about HPV-related carcinogenesis in HNSCC. The purpose of this study is to characterize immortalized human oral keratinocyte (IHOK) transfected by HPV16 E6/E7, IHOK/hcdk4 (IHOK transfected by pLXRN-hcdk4) and IHOK/hcdk4/hTERT (IHOK transfected by pLPC-hTERT-hcdk4) to reconstitute HNSCC in vitro. Conclusively, we established a new immortalized cell lines, IHOK/hcdk4 and IHOK/hcdk4/hTERT, to understand multistep carcinogenic process of oncogenic HPV16 E6/E7 in HNSCC.
키워드
hcdk4; HPV16 E6/E7; hTERT; Immortalization
원문 및 링크아웃 정보
등재저널 정보